Loss of SNAP29 impairs endocytic recycling and cell motility.

ABSTRACT: Intracellular membrane trafficking depends on the ordered formation and consumption of transport intermediates and requires that membranes fuse with each other in a tightly regulated and highly specific manner. Membrane anchored SNAREs assemble into SNARE complexes that bring membranes together to promote fusion. SNAP29 is a ubiquitous synaptosomal-associated SNARE protein. It interacts with several syntaxins and with the EH domain containing protein EHD1. Loss of functional SNAP29 results in CEDNIK syndrome (Cerebral Dysgenesis, Neuropathy, Ichthyosis and Keratoderma). Using fibroblast cell lines derived from CEDNIK patients, we show that SNAP29 mediates endocytic recycling of transferrin and beta1-integrin. Impaired beta1-integrin recycling affected cell motility, as reflected by changes

SUBMITTER: Rapaport D 

PROVIDER: S-EPMC2841205 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature