Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo.

ABSTRACT:

Background

The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples. Additionally, cartilaginous tissues are characterized by a low cellularity and an abundance of extracellular matrix (ECM) molecules. But given the growing interest in understanding pathogenesis of degenerative diseases, e.g. osteoarthritis (OA) and rheumatoid arthritis (RA), studies have to consider expression pattern of cells in its natural environment.

Findings

We compared the current RNA isolation methods for the extraction of high-quality RNA of snap-frozen biopsies from limited amounts of hypocellular cartilaginous tissue. The focus of the study was to gather information about procedure-related differences in

SUBMITTER: Ruettger A 

PROVIDER: S-EPMC2841606 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature