Variability of gene expression profiles in human blood and lymphoblastoid cell lines.

ABSTRACT:

Background

Readily accessible samples such as peripheral blood or cell lines are increasingly being used in large cohorts to characterise gene expression differences between a patient group and healthy controls. However, cell and RNA isolation procedures and the variety of cell types that make up whole blood can affect gene expression measurements. We therefore systematically investigated global gene expression profiles in peripheral blood from six individuals collected during two visits by comparing five of the following cell and RNA isolation methods: whole blood (PAXgene), peripheral blood mononuclear cells (PBMCs), lymphoblastoid cell lines (LCLs), CD19 and CD20 specific B-cell subsets.

Results

Gene expression measurements were clearly discriminated by isolation method a

SUBMITTER: Min JL 

PROVIDER: S-EPMC2841682 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature