Intronic CArG box regulates cysteine-rich protein 2 expression in the adult but not in developing vasculature.

ABSTRACT:

Objective

An absence of cysteine-rich protein 2 (CRP2) enhances vascular smooth muscle cell (VSMC) migration and increases neointima formation after arterial injury; therefore, CRP2 plays an important role in the response to vascular injury. The goal of the present study was to elucidate the molecular mechanisms that preserve CRP2 expression in the adult vasculature and thus might serve to inhibit the response to injury.

Methods and results

We generated a series of transgenic mice harboring potential Csrp2 regulatory regions with a lacZ reporter. We determined that the 12-kb first intron was necessary for transgene activity in adult but not in developing vasculature. Within the intron we identified a 6.3-kb region that contains 2 CArG boxes. Serum response factor preferentia

SUBMITTER: Chen CH 

PROVIDER: S-EPMC2841712 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature