In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands.

ABSTRACT: In vivo fluorescence cancer imaging is an important tool in understanding tumor growth and therapeutic monitoring and can be performed either with endogenously produced fluorescent proteins or with exogenously introduced fluorescent probes bound to targeting molecules. However, endogenous fluorescence proteins cannot be altered after transfection, thus requiring rederivation of cell lines for each desired color, while exogenously targeted fluorescence probes are limited by the heterogeneous expression of naturally occurring cellular targets. In this study, we adapted the dehalogenase-based protein-Tag (HaloTag) system to in vivo cancer imaging, by introducing highly expressed HaloTag receptors (HaloTagR) in cancer cells coupled with a range of externally injected fluorophore-conjugated deh

SUBMITTER: Kosaka N 

PROVIDER: S-EPMC2841743 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature