Partitioning of perfluorooctanoate into phosphatidylcholine bilayers is chain length-independent.

ABSTRACT: The chain length dependence of the interaction of PFOA, a persistent environmental contaminant, with dimyristoyl- (DMPC), dipalmitoyl- (DPPC) and distearoylphosphatidylcholine (DSPC) was investigated using steady-state fluorescence anisotropy spectroscopy, differential scanning calorimetry (DSC) and dynamic light scattering (DLS). PFOA caused a linear depression of the main phase transition temperature T(m) while increasing the width of the phase transition of all three phosphatidylcholines. Although PFOA's effect on T(m) and the transition width decreased in the order DMPC>DPPC>DSPC, its relative effect on the phase behavior was largely independent of the phosphatidylcholine. PFOA caused swelling of DMPC but not DPPC and DSPC liposomes at 37 degrees C in the DLS experiments, which suggest

SUBMITTER: Xie W 

PROVIDER: S-EPMC2841784 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature