Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots.

ABSTRACT:

Background

Proteomics is increasingly becoming an important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying in plant physiology, development, differentiation and their interaction with the environments. To investigate the cassava (Manihot esculenta Crantz) proteome, we extracted proteins from somatic embryos, plantlets and tuberous roots of cultivar SC8 and separated them by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Results

Analysis by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) yielded a total of 383 proteins including isoforms, classified into 14 functional groups. The majority of these were carbohydrate and energy metabolism assoc

SUBMITTER: Li K 

PROVIDER: S-EPMC2842255 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature