A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.

ABSTRACT: We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.

SUBMITTER: Schwarz F 

PROVIDER: S-EPMC2842479 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature