DJ-1 and alpha-synuclein in human cerebrospinal fluid as biomarkers of Parkinson's disease.

ABSTRACT: Biomarkers are urgently needed for the diagnosis and monitoring of disease progression in Parkinson's disease. Both DJ-1 and alpha-synuclein, two proteins critically involved in Parkinson's disease pathogenesis, have been tested as disease biomarkers in several recent studies with inconsistent results. These have been largely due to variation in the protein species detected by different antibodies, limited numbers of patients in some studies, or inadequate control of several important variables. In this study, the nature of DJ-1 and alpha-synuclein in human cerebrospinal fluid was studied by a combination of western blotting, gel filtration and mass spectrometry. Sensitive and quantitative Luminex assays detecting most, if not all, species of DJ-1 and alpha-synuclein in human cerebrospinal

SUBMITTER: Hong Z 

PROVIDER: S-EPMC2842513 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature