LC/MS analysis of cellular RNA reveals NAD-linked RNA.

ABSTRACT: We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell.

SUBMITTER: Chen YG 

PROVIDER: S-EPMC2842606 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature