Development of pooled suppression subtractive hybridization to analyze the pangenome of Staphylococcus aureus.

ABSTRACT: We describe the development and application of a Pooled Suppression Subtractive Hybridization (PSSH) method to describe differences between the genomic content of a pool of clinical Staphylococcus aureus isolates and a sequenced reference strain. In comparative bacterial genomics, Suppression Subtractive Hybridization (SSH) is normally utilized to compare genomic features or expression profiles of one strain versus another, which limits its ability to analyze communities of isolates. However, a PSSH approach theoretically enables the user to characterize the entirety of gene content unique to a related group of isolates in a single reaction. These unique fragments may then be linked to individual isolates through standard PCR. This method was applied to examine the genomic diversity found

SUBMITTER: Gerrish RS 

PROVIDER: S-EPMC2843094 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature