Physical interaction of Jab1 with human serotonin 6 G-protein-coupled receptor and their possible roles in cell survival.

ABSTRACT: The 5-HT(6) receptor (5-HT(6)R) is one of the most recently cloned serotonin receptors, and it plays important roles in Alzheimer disease, depression, and learning and memory disorders. However, unlike the other serotonin receptors, the cellular mechanisms of 5-HT(6)R are poorly elucidated relative to its significance in human brain diseases. Here, using a yeast two-hybrid assay, we found that the human 5-HT(6)R interacts with Jun activation domain-binding protein-1 (Jab1). We also confirmed a physical interaction between 5-HT(6)R and Jab1 using glutathione S-transferase pulldown, fluorescence resonance energy transfer, co-immunoprecipitation, and immunocyto(histo)chemistry assays. The manipulation of Jab1 expression using Jab1 small interference RNA decreased 5-HT(6)R-mediated activity an

SUBMITTER: Yun HM 

PROVIDER: S-EPMC2843165 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature