From QTL to candidate gene: genetical genomics of simple and complex traits in potato using a pooling strategy.

ABSTRACT:

Background

Utilization of the natural genetic variation in traditional breeding programs remains a major challenge in crop plants. The identification of candidate genes underlying, or associated with, phenotypic trait QTLs is desired for effective marker assisted breeding. With the advent of high throughput -omics technologies, screening of entire populations for association of gene expression with targeted traits is becoming feasible but remains costly. Here we present the identification of novel candidate genes for different potato tuber quality traits by employing a pooling approach reducing the number of hybridizations needed. Extreme genotypes for a quantitative trait are collected and the RNA from contrasting bulks is then profiled with the aim of finding differentially expre

SUBMITTER: Kloosterman B 

PROVIDER: S-EPMC2843620 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature