Constrained solution scattering modelling of human antibodies and complement proteins reveals novel biological insights.

ABSTRACT: X-ray and neutron-scattering techniques characterize proteins in solution and complement high-resolution structural studies. They are useful when either a large protein cannot be crystallized, in which case scattering yields a solution structure, or a crystal structure has been determined and requires validation in solution. These solution structures are determined by the application of constrained modelling methods based on known subunit structures. First, an appropriate starting model is generated. Next, its conformation is randomized to generate thousands of models for trial-and-error fits. Comparison with the experimental data identifies a small family of best-fit models. Finally, their significance for biological function is assessed. We illustrate this in application to structure det

SUBMITTER: Perkins SJ 

PROVIDER: S-EPMC2843969 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature