The G-protein-coupled formylpeptide receptor FPR confers a more invasive phenotype on human glioblastoma cells.

ABSTRACT:

Background

The G-protein-coupled formylpeptide receptor (FPR) that mediates chemotaxis of phagocytic leucocytes induced by bacterial and host-derived chemotactic peptides is selectively expressed by highly malignant human gliomas and contributes to tumour growth and angiogenesis. As invasion of surrounding normal tissues is one of the important features of tumour malignancy, we investigated the function of FPR in the invasive behaviour of human glioblastoma cells.

Methods

Cells (FPR(+) and FPR(-)) were isolated by single-cell cloning from a human glioblastoma cell line U-87MG. The FPR expression was assayed by flow cytometry and reverse transcription PCR. The function of FPR was investigated by chemotaxis and calcium flux induced by FPR agonist fMLF. Tumour cell motility was

SUBMITTER: Huang J 

PROVIDER: S-EPMC2844039 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature