Discovery of Phytophthora infestans genes expressed in planta through mining of cDNA libraries.

ABSTRACT:

Background

Phytophthora infestans (Mont.) de Bary causes late blight of potato and tomato, and has a broad host range within the Solanaceae family. Most studies of the Phytophthora--Solanum pathosystem have focused on gene expression in the host and have not analyzed pathogen gene expression in planta.

Methodology/principal findings

We describe in detail an in silico approach to mine ESTs from inoculated host plants deposited in a database in order to identify particular pathogen sequences associated with disease. We identified candidate effector genes through mining of 22,795 ESTs corresponding to P. infestans cDNA libraries in compatible and incompatible interactions with hosts from the Solanaceae family.

Conclusions/significance

We annotated genes of P. infestans e

SUBMITTER: Sierra R 

PROVIDER: S-EPMC2844423 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature