High glucose-induced oxidative stress increases transient receptor potential channel expression in human monocytes.

ABSTRACT:

Objective

Transient receptor potential (TRP) channel-induced cation influx activates human monocytes, which play an important role in the pathogenesis of atherosclerosis. In the present study, we investigated the effects of high glucose-induced oxidative stress on TRP channel expression in human monocytes.

Research design and methods

Human monocytes were exposed to control conditions (5.6 mmol/l d-glucose), high glucose (30 mmol/l d-glucose or l-glucose), 100 micromol/l peroxynitrite, or high glucose in the presence of the superoxide dismutase mimetic tempol (100 micromol/l). TRP mRNA and TRP protein expression was measured using quantitative real-time RT-PCR and quantitative in-cell Western assay, respectively. Calcium influx and intracellular reactive oxygen species were m

SUBMITTER: Wuensch T 

PROVIDER: S-EPMC2844832 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature