Glucose levels at the site of subcutaneous insulin administration and their relationship to plasma levels.

ABSTRACT:

Objective

To examine insulin's effect on the tissue glucose concentration at the site of subcutaneous insulin administration.

Research design and methods

A CMA-60 microdialysis (MD) catheter and a 24-gauge microperfusion (MP) catheter were inserted into the subcutaneous adipose tissue of fasting, healthy subjects (n = 5). Both catheters were perfused with regular human insulin (100 units/ml) over a 6-h period and used for glucose sampling and simultaneous administration of insulin at sequential rates of 0.33, 0.66, and 1.00 units/h (each rate was used for 2 h). Before and after the insulin delivery period, both catheters were perfused with an insulin-free solution (5% mannitol) for 2 h and used for glucose sampling only. Blood plasma glucose was clamped at euglycemic levels

SUBMITTER: Lindpointner S 

PROVIDER: S-EPMC2845037 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature