Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum.

ABSTRACT:

Background

The extracellular promastigote and the intracellular amastigote stages alternate in the digenetic life cycle of the trypanosomatid parasite Leishmania. Amastigotes develop inside parasitophorous vacuoles of mammalian phagocytes, where they tolerate extreme environmental conditions. Temperature increase and pH decrease are crucial factors in the multifactorial differentiation process of promastigotes to amastigotes. Although expression profiling approaches for axenic, cell culture- and lesion-derived amastigotes have already been reported, the specific influence of temperature increase and acidification of the environment on developmental regulation of genes has not been previously studied. For the first time, we have used custom L. infantum genomic DNA microarrays to com

SUBMITTER: Alcolea PJ 

PROVIDER: S-EPMC2845110 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature