Serial bone marrow transplantation reveals in vivo expression of the pCLPG retroviral vector.

ABSTRACT:

Background

Gene therapy in the hematopoietic system remains promising, though certain aspects of vector design, such as transcriptional control elements, continue to be studied. Our group has developed a retroviral vector where transgene expression is controlled by p53 with the intention of harnessing the dynamic and inducible nature of this tumor suppressor and transcription factor. We present here a test of in vivo expression provided by the p53-responsive vector, pCLPG. For this, we used a model of serial transplantation of transduced bone marrow cells.

Results

We observed, by flow cytometry, that the eGFP transgene was expressed at higher levels when the pCLPG vector was used as compared to the parental pCL retrovirus, where expression is directed by the native MoMLV LTR

SUBMITTER: Fratini P 

PROVIDER: S-EPMC2845565 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature