RNA isolation method for single embryo transcriptome analysis in zebrafish.

ABSTRACT:

Background

Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variation among individuals is confounded. This can irreversibly reduce the robustness, resolution, or expressiveness of the experiment. Therefore, we developed a robust method to isolate abundant high-quality RNA from individual embryos to perform single embryo transcriptome analyses using zebrafish as a model organism. Available methods for embryonic zebrafish RNA isolation minimally utilize ten embryos. Further downscaling of these methods to one embryo is practically not feasible.

Findings

We developed a single embryo RNA extraction method b

SUBMITTER: de Jong M 

PROVIDER: S-EPMC2845602 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature