Allele-specific regulation of matrix metalloproteinase-3 gene by transcription factor NFkappaB.

ABSTRACT:

Background

Matrix metalloproteinase-3 (MMP3) is implicated in the pathogenesis and progression of atherosclerotic lesions. Previous studies suggested that MMP3 expression is influenced by a polymorphism (known as the 5A/6A polymorphism) in the promoter of the MMP3 gene and that this polymorphism is located within a cis-element that interacts with the transcription factor NFkappaB. In the present study, we sought to investigate whether MMP3 and NFkappaB were co-localized in atherosclerotic lesions and whether NFkappaB had differential effects on the two alleles of the MMP3 5A/6A polymorphism.

Methodology/principal findings

Immunohistochemical examination showed that MMP3 and both the NFkappaB p50 and p65 subunits were expressed abundantly in macrophages in atherosclerotic les

SUBMITTER: Souslova V 

PROVIDER: S-EPMC2845631 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature