Community analysis of betaproteobacterial ammonia-oxidizing bacteria using the amoCAB operon.

ABSTRACT: The genes and intergenic regions of the amoCAB operon were analyzed to establish their potential as molecular markers for analyzing ammonia-oxidizing betaproteobacterial (beta-AOB) communities. Initially, sequence similarity for related taxa, evolutionary rates from linear regressions, and the presence of conserved and variable regions were analyzed for all available sequences of the complete amoCAB operon. The gene amoB showed the highest sequence variability of the three amo genes, suggesting that it might be a better molecular marker than the most frequently used amoA to resolve closely related AOB species. To test the suitability of using the amoCAB genes for community studies, a strategy involving nested PCR was employed. Primers to amplify the whole amoCAB operon and each individual

SUBMITTER: Junier P 

PROVIDER: S-EPMC2845890 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature