Longitudinal-relaxation-enhanced NMR experiments for the study of nucleic acids in solution.

ABSTRACT: Atomic-resolution information on the structure and dynamics of nucleic acids is essential for a better understanding of the mechanistic basis of many cellular processes. NMR spectroscopy is a powerful method for studying the structure and dynamics of nucleic acids; however, solution NMR studies are currently limited to relatively small nucleic acids at high concentrations. Thus, technological and methodological improvements that increase the experimental sensitivity and spectral resolution of NMR spectroscopy are required for studies of larger nucleic acids or protein-nucleic acid complexes. Here we introduce a series of imino-proton-detected NMR experiments that yield an over 2-fold increase in sensitivity compared to conventional pulse schemes. These methods can be applied to the detecti

SUBMITTER: Farjon J 

PROVIDER: S-EPMC2846706 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature