Effects of scanning sensitivity and multiple scan algorithms on microarray data quality.

ABSTRACT:

Background

Maximizing the utility of DNA microarray data requires optimization of data acquisition through selection of an appropriate scanner setting. To increase the amount of useable data, several approaches have been proposed that incorporate multiple scans at different sensitivities to reduce the quantification error and to minimize effects of saturation. However, no direct comparison of their efficacy has been made. In the present study we compared individual scans at low, medium and high sensitivity with three methods for combining data from multiple scans (either 2-scan or 3-scan cases) using an actual dataset comprising 40 technical replicates of a reference RNA standard.

Results

Of the individual scans, the low scan exhibited the lowest background signal, the highe

SUBMITTER: Williams A 

PROVIDER: S-EPMC2846908 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature