Cocaine- and amphetamine-regulated transcript mediates the actions of cholecystokinin on rat vagal afferent neurons.

ABSTRACT:

Background & aims

Cholecystokinin (CCK) acts on vagal afferent neurons to inhibit food intake and gastric emptying; it also increases expression of the neuropeptide cocaine- and amphetamine-regulated transcript (CART), but the significance of this is unknown. We investigated the role of CARTp in vagal afferent neurons.

Methods

Release of CART peptide (CARTp) from cultured vagal afferent neurons was determined by enzyme-linked immunosorbent assay. Expression of receptors and neuropeptides in rat vagal afferent neurons in response to CARTp was studied using immunohistochemistry and luciferase promoter reporter constructs. Effects of CARTp and CCK were studied on food intake.

Results

CCK stimulated CARTp release from cultured nodose neurons. CARTp replicated the effect o

SUBMITTER: De Lartigue G 

PROVIDER: S-EPMC2847060 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature