Probing intermediates in the activation cycle of [NiFe] hydrogenase by infrared spectroscopy: the Ni-SIr state and its light sensitivity.

ABSTRACT: The [NiFe] hydrogenase from the sulphate-reducing bacterium Desulfovibrio vulgaris Miyazaki F is reversibly inhibited in the presence of molecular oxygen. A key intermediate in the reactivation process, Ni-SI(r), provides the link between fully oxidized (Ni-A, Ni-B) and active (Ni-SI(a), Ni-C and Ni-R) forms of hydrogenase. In this work Ni-SI(r) was found to be light-sensitive (T

SUBMITTER: Pandelia ME 

PROVIDER: S-EPMC2847147 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature