O Labeling for a Quantitative Proteomic Analysis of Glycoproteins in Hepatocellular Carcinoma.

ABSTRACT: INTRODUCTION: Quantitative proteomics using tandem mass spectrometry is an attractive approach for identification of potential cancer biomarkers. Fractionation of complex tissue samples into subproteomes prior to mass spectrometric analyses increases the likelihood of identifying cancer-specific proteins that might be present in low abundance. In this regard, glycosylated proteins are an interesting class of proteins that are already established as biomarkers for several cancers. MATERIALS AND METHODS: In this study, we carried out proteomic profiling of tumor and adjacent non-cancer liver tissues from hepatocellular carcinoma (HCC) patients. Glycoprotein enrichment from liver samples using lectin affinity chromatography and subsequent (18)O/(16)O labeling of peptides allowed us to obtain

SUBMITTER: Chaerkady R 

PROVIDER: S-EPMC2847390 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature