Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts.

ABSTRACT:

Background

Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome repositioning is elicited.

Results

By investigating the positioning of all human chromosomes in primary fibroblasts that have left the proliferative cell cycle, we have demonstrated that in cells made quiescent by reversible growth arrest, chromosome positioning is altered considerably. We found that with the removal of serum from the culture medium, chromosome repositioning took less than 15 minutes, required energy and was inhibited by drugs affecting the polymerization of myosin and actin. We also observed that when cells became quiescent, the nuclear dis

SUBMITTER: Mehta IS 

PROVIDER: S-EPMC2847717 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature