RNA-binding protein quaking, a critical regulator of colon epithelial differentiation and a suppressor of colon cancer.

ABSTRACT:

Background & aims

Colon cancer is one of the best understood neoplasms from a genetic perspective, yet it remains the second most common cause of cancer-related death. Post-transcriptional regulation mediated by RNA-binding proteins or microRNAs coordinately targets multiple genes, holding promise involved in colon cancer initiation and development. Here we studied the role of RNA-binding protein quaking (QKI) in colon cancer.

Methods

We observed the expression pattern of QKI in normal colon and colon cancers through reverse-transcription polymerase chain reaction and Western blot. Bisulfite sequencing and methylation-specific PCR were applied for QKI promoter methylation analysis. We used enterocyte differentiation markers and soft agar assay to test the role of QKI in colo

SUBMITTER: Yang G 

PROVIDER: S-EPMC2847771 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature