2D-DIGE proteomic characterization of head and neck squamous cell carcinoma.

ABSTRACT:

Objective

Identify proteins that are differentially expressed between head and neck squamous cell cancer (HNSCC) and patient-matched normal adjacent tissue, and validate findings in a separate patient cohort.

Study design

Cross-sectional study of surgical specimens.

Setting

Tertiary care academic medical center.

Subjects and methods

Laser capture microdissection and two-dimensional difference gel electrophoresis were used previously to establish proteomic profiles for tumor and normal adjacent tissue from 14 patients. Here, significance analysis of microarray was used to rank candidate biomarkers. Spots meeting statistical and biological criteria of significance were analyzed by liquid chromatography and tandem mass spectrometry to obtain protein identification

SUBMITTER: Merkley MA 

PROVIDER: S-EPMC2847880 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature