Insulation of a synthetic hydrogen metabolism circuit in bacteria.

ABSTRACT:

Background

The engineering of metabolism holds tremendous promise for the production of desirable metabolites, particularly alternative fuels and other highly reduced molecules. Engineering approaches must redirect the transfer of chemical reducing equivalents, preventing these electrons from being lost to general cellular metabolism. This is especially the case for high energy electrons stored in iron-sulfur clusters within proteins, which are readily transferred when two such clusters are brought in close proximity. Iron sulfur proteins therefore require mechanisms to ensure interaction between proper partners, analogous to many signal transduction proteins. While there has been progress in the isolation of engineered metabolic pathways in recent years, the design of insulated el

SUBMITTER: Agapakis CM 

PROVIDER: S-EPMC2847965 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature