Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum.

ABSTRACT:

Background

Little genomic or trancriptomic information on Ganoderma lucidum (Lingzhi) is known. This study aims to discover the transcripts involved in secondary metabolite biosynthesis and developmental regulation of G. lucidum using an expressed sequence tag (EST) library.

Methods

A cDNA library was constructed from the G. lucidum fruiting body. Its high-quality ESTs were assembled into unique sequences with contigs and singletons. The unique sequences were annotated according to sequence similarities to genes or proteins available in public databases. The detection of simple sequence repeats (SSRs) was preformed by online analysis.

Results

A total of 1,023 clones were randomly selected from the G. lucidum library and sequenced, yielding 879 high-quality ESTs. These

SUBMITTER: Luo H 

PROVIDER: S-EPMC2848221 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature