Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feeding.

ABSTRACT:

Background

The hypothalamus plays a central role in regulating appetite and metabolism. However, the gene networks within the hypothalamus that regulate feed intake and metabolism, and the effects of fasting on those pathways are not completely understood in any species. The present experiment evaluated global hypothalamic gene expression in newly hatched chicks using microarray analysis to elucidate genes and pathways regulated by feeding, fasting, and delayed feeding. Ten groups of chicks were sampled over four days post-hatch, including fed, fasted, and 48 h fasted followed by access to feed for 4 h, 24 h, and 48 h. Hypothalamic samples were collected for microarray analysis (n = 4). Expression patterns of selected genes were confirmed by quantitative real-time PCR. Pathway anal

SUBMITTER: Higgins SE 

PROVIDER: S-EPMC2848243 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature