CpG-enhanced CD8+ T-cell responses to peptide immunization are severely inhibited by B cells.

ABSTRACT: Synthetic peptides encoding protective pathogen-derived epitopes represent--in principle--an ideal approach to T-cell vaccination. Empirically, however, these strategies have not been successful. In the current study, we profiled the early activation of CD8+ T cells by MHC class I-restricted peptide immunization to better understand the biology of this response. We found that CD8+ T cells proliferated robustly in response to low doses of short synthetic peptides in PBS, but failed to acquire effector function or form memory populations in the absence of the TLR ligand CpG. CpG was unique among TLR ligands in its ability to enhance the response to peptide and its adjuvant effects had strict temporal requirements. Interestingly, CpG treatment modulated T-cell expression of the surface recept

SUBMITTER: Overstreet MG 

PROVIDER: S-EPMC2848446 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature