A modifier screen for Bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium.

ABSTRACT:

Background

The development and homeostasis of multicellular organisms depends on sheets of epithelial cells. Bazooka (Baz; PAR-3) localizes to the apical circumference of epithelial cells and is a key hub in the protein interaction network regulating epithelial structure. We sought to identify additional proteins that function with Baz to regulate epithelial structure in the Drosophila embryo.

Methodology/principal findings

The baz zygotic mutant cuticle phenotype could be dominantly enhanced by loss of known interaction partners. To identify additional enhancers, we screened molecularly defined chromosome 2 and 3 deficiencies. 37 deficiencies acted as strong dominant enhancers. Using deficiency mapping, bioinformatics, and available single gene mutations, we identified 17 i

SUBMITTER: Shao W 

PROVIDER: S-EPMC2848566 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature