Differential changes of glutathione levels in astrocytes and neurons in ischemic brains by two-photon imaging.
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ABSTRACT:
SUBMITTER: Bragin DE
PROVIDER: S-EPMC2848708 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Publications
Differential changes of glutathione levels in astrocytes and neurons in ischemic brains by two-photon imaging.
Bragin Denis E DE Zhou Bo B Ramamoorthy Prabhu P Müller Wolfgang S WS Connor John A JA Shi Honglian H
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20100127 4
Using two-photon imaging techniques with monochlorobimane as a glutathione (GSH) probe, we investigated GSH levels in both core and penumbra regions of an ischemic brain after middle cerebral artery occlusion. We found that the GSH level significantly decreased in the ischemic core, but increased significantly in the penumbra. Furthermore, we observed a differential change of the GSH levels in neurons and astrocytes in the penumbra. The GSH level in neurons increased significantly whereas it dec ...[more]
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