Delta-aminolevulinic acid is a substrate for the amino acid transporter SLC36A1 (hPAT1).

ABSTRACT:

Background and purpose

delta-Aminolevulinic acid (ALA) is used in cancer patients for photodynamic diagnosis or therapy. Oral administration of ALA has been used in patients with prostate and bladder cancer. The present aim was to investigate the mechanism of intestinal absorption of ALA and its transport via the amino acid transporter SLC36A1.

Experimental approach

In vitro investigations of ALA affinity for and uptake via SLC36A1 and SLC15A1 were performed in Caco-2 cell monolayers. Interaction of ALA with SLC15A1 was investigated in MDCK/SLC15A1 cells, whereas interactions with SLC36A1 were investigated in COS-7 cells transiently expressing SLC36A1.

Key results

ALA inhibited SLC36A1-mediated L-[(3)H]Pro and SLC15A1-mediated [(14)C]Gly-Sar uptake in Caco-2 cell mono

SUBMITTER: Frolund S 

PROVIDER: S-EPMC2848937 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature