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New efficient recombinant expression system to engineer Candida antarctica lipase B.

ABSTRACT: Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.

SUBMITTER: Emond S 

PROVIDER: S-EPMC2849202 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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New efficient recombinant expression system to engineer Candida antarctica lipase B.

Emond Stéphane S   Montanier Cédric C   Nicaud Jean-Marc JM   Marty Alain A   Monsan Pierre P   André Isabelle I   Remaud-Siméon Magali M  

Applied and environmental microbiology 20100219 8

Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme. ...[more]

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