High abundance of ammonia-oxidizing Archaea in coastal waters, determined using a modified DNA extraction method.

ABSTRACT: Molecular characterizations of environmental microbial populations based on recovery and analysis of DNA generally assume efficient or unbiased extraction of DNA from different sample matrices and microbial groups. Appropriate controls to verify this basic assumption are rarely included. Here three different DNA extractions, performed with two commercial kits (FastDNA and UltraClean) and a standard phenol-chloroform method, and two alternative filtration methods (Sterivex and 25-mm-diameter polycarbonate filters) were evaluated, using the addition of Nitrosopumilus maritimus cells to track the recovery of DNA from marine Archaea. After the comparison, a simplified phenol-chloroform extraction method was developed and shown to be significantly superior, in terms of both the recovery and the

SUBMITTER: Urakawa H 

PROVIDER: S-EPMC2849251 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature