Downregulation of connective tissue growth factor by three-dimensional matrix enhances ovarian carcinoma cell invasion.

ABSTRACT: Epithelial ovarian carcinoma (EOC) is a leading cause of death from gynecologic malignancies, due mainly to the prevalence of undetected metastatic disease. The process of cell invasion during intraperitoneal anchoring of metastatic lesions requires concerted regulation of many processes, including modulation of adhesion to the extracellular matrix and localized invasion. Exploratory cDNA microarray analysis of early response genes (altered after 4 hr of 3D collagen culture) coupled with confirmatory real-time reverse-transcriptase polymerase chain reaction, multiple 3D cell culture matrices, Western blot, immunostaining, adhesion, migration and invasion assays were used to identify modulators of adhesion pertinent to EOC progression and metastasis. cDNA microarray analysis indicated a dra

SUBMITTER: Barbolina MV 

PROVIDER: S-EPMC2849282 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature