Genetic dissection of azole resistance mechanisms in Candida albicans and their validation in a mouse model of disseminated infection.

ABSTRACT: Principal mechanisms of resistance to azole antifungals include the upregulation of multidrug transporters and the modification of the target enzyme, a cytochrome P450 (Erg11) involved in the 14alpha-demethylation of ergosterol. These mechanisms are often combined in azole-resistant Candida albicans isolates recovered from patients. However, the precise contributions of individual mechanisms to C. albicans resistance to specific azoles have been difficult to establish because of the technical difficulties in the genetic manipulation of this diploid species. Recent advances have made genetic manipulations easier, and we therefore undertook the genetic dissection of resistance mechanisms in an azole-resistant clinical isolate. This isolate (DSY296) upregulates the multidrug transporter genes

SUBMITTER: MacCallum DM 

PROVIDER: S-EPMC2849354 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature