Antibodies fused to innate immune molecules reduce initiation of Cryptosporidium parvum infection in mice.

ABSTRACT: At present no completely effective treatments are available for Cryptosporidium parvum infections in humans and livestock. Based on previous data showing the neutralizing potential of a panel of monoclonal antibodies developed against C. parvum, and based on the fact that innate immune peptides and enzymes have anticryptosporidial activity, we engineered several of these antibodies into antibody-biocide fusion proteins. We hypothesized that the combination of high-affinity antibody targeting with innate immune molecule-mediated killing would result in a highly effective new antiprotozoal agent. To test this hypothesis, we expressed antibody-biocide fusion proteins in a mammalian cell culture system and used the resulting products for in vitro and in vivo efficacy experiments. Antibody-bioc

SUBMITTER: Imboden M 

PROVIDER: S-EPMC2849385 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature