Analysis of protein expression regulated by the Helicobacter pylori ArsRS two-component signal transduction system.

ABSTRACT: Previous studies have shown that the Helicobacter pylori ArsRS two-component signal transduction system contributes to acid-responsive gene expression. To identify additional members of the ArsRS regulon and further investigate the regulatory role of the ArsRS system, we analyzed protein expression in wild-type and arsS null mutant strains. Numerous proteins were differentially expressed in an arsS mutant strain compared to a wild-type strain when the bacteria were cultured at pH 5.0 and also when they were cultured at pH 7.0. Genes encoding 14 of these proteins were directly regulated by the ArsRS system, based on observed binding of ArsR to the relevant promoter regions. The ArsRS-regulated proteins identified in this study contribute to acid resistance (urease and amidase), acetone meta

SUBMITTER: Loh JT 

PROVIDER: S-EPMC2849440 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature