Ontology highlight
ABSTRACT:
SUBMITTER: Wen X
PROVIDER: S-EPMC2849461 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Wen Xiaohui X Lai Cary K CK Evangelista Marie M Hongo Jo-Anne JA de Sauvage Frederic J FJ Scales Suzie J SJ
Molecular and cellular biology 20100212 8
Hedgehog (Hh) signaling in vertebrates depends on intraflagellar transport (IFT) within primary cilia. The Hh receptor Patched is found in cilia in the absence of Hh and is replaced by the signal transducer Smoothened within an hour of Hh stimulation. By generating antibodies capable of detecting endogenous pathway transcription factors Gli2 and Gli3, we monitored their kinetics of accumulation in cilia upon Hh stimulation. Localization occurs within minutes of Hh addition, making it the fastest ...[more]