Glycation isotopic labeling with 13C-reducing sugars for quantitative analysis of glycated proteins in human plasma.

ABSTRACT: Non-enzymatic glycation of proteins is a post-translational modification produced by a reaction between reducing sugars and amino groups located in lysine and arginine residues or in the N-terminal position. This modification plays a relevant role in medicine and food industry. In the clinical field, this undesired role is directly linked to blood glucose concentration and therefore to pathological conditions derived from hyperglycemia (>11 mm glucose) such as diabetes mellitus or renal failure. An approach for qualitative and quantitative analysis of glycated proteins is here proposed to achieve the three information levels for their complete characterization. These are: 1) identification of glycated proteins, 2) elucidation of sugar attachment sites, and 3) quantitative analysis to compa

SUBMITTER: Priego-Capote F 

PROVIDER: S-EPMC2849708 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature