Analysis of chemotherapy response programs in ovarian cancers by the next-generation sequencing technologies.

ABSTRACT:

Objective

To understand the chemotherapy response program in ovarian cancer cells at deep transcript sequencing levels.

Methods

Two next-generation sequencing technologies--MPSS (massively parallel signature sequencing) and SBS (sequencing by synthesis)--were used to sequence the transcripts of IGROV1 and IGROV1-CP cells, and to sequence the transcripts of a highly chemotherapy responsive and a highly chemotherapy resistant ovarian cancer tissue.

Results

We identified 3422 signatures (2957 genes) that are significantly different between IGROV1 and IGROV1-CP cells (P<0.001). Gene Ontology (GO) term GO:0001837 (epithelial-to-mesenchymal transition) and GO:0034330 (cell junction assembly and maintenance) are enriched in genes that are over expressed in IGROV1-CP cells wh

SUBMITTER: Cheng L 

PROVIDER: S-EPMC2849907 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature