Increased throughput by parallelization of library preparation for massive sequencing.

ABSTRACT:

Background

Massively parallel sequencing systems continue to improve on data output, while leaving labor-intensive library preparations a potential bottleneck. Efforts are currently under way to relieve the crucial and time-consuming work to prepare DNA for high-throughput sequencing.

Methodology/principal findings

In this study, we demonstrate an automated parallel library preparation protocol using generic carboxylic acid-coated superparamagnetic beads and polyethylene glycol precipitation as a reproducible and flexible method for DNA fragment length separation. With this approach the library preparation for DNA sequencing can easily be adjusted to a desired fragment length. The automated protocol, here demonstrated using the GS FLX Titanium instrument, was compared to the

SUBMITTER: Lundin S 

PROVIDER: S-EPMC2850305 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature