Inverse agonist and neutral antagonist actions of synthetic compounds at an insect 5-HT1 receptor.

ABSTRACT:

Background and purpose

5-Hydroxytryptamine (5-HT) has been shown to control and modulate many physiological and behavioural functions in insects. In this study, we report the cloning and pharmacological properties of a 5-HT(1) receptor of an insect model for neurobiology, physiology and pharmacology.

Experimental approach

A cDNA encoding for the Periplaneta americana 5-HT(1) receptor was amplified from brain cDNA. The receptor was stably expressed in HEK 293 cells, and the functional and pharmacological properties were determined in cAMP assays. Receptor distribution was investigated by RT-PCR and by immunocytochemistry using an affinity-purified polyclonal antiserum.

Key results

The P. americana 5-HT(1) receptor (Pea5-HT(1)) shares pronounced sequence and functional

SUBMITTER: Troppmann B 

PROVIDER: S-EPMC2850402 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature