Alpha-synuclein overexpression increases dopamine toxicity in BE2-M17 cells.

ABSTRACT:

Background

Oxidative stress has been proposed to be involved in the pathogenesis of Parkinson's disease (PD). A plausible source of oxidative stress in nigral dopaminergic neurons is the redox reactions that specifically involve dopamine and produce various toxic molecules, i.e., free radicals and quinone species. alpha-Synuclein, a protein found in Lewy bodies characteristic of PD, is also thought to be involved in the pathogenesis of PD and point mutations and multiplications in the gene coding for alpha-synuclein have been found in familial forms of PD.

Results

We used dopaminergic human neuroblastoma BE(2)-M17 cell lines stably transfected with WT or A30P mutant alpha-synuclein to characterize the effect of alpha-synuclein on dopamine toxicity. Cellular toxicity was anal

SUBMITTER: Bisaglia M 

PROVIDER: S-EPMC2851596 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature